Microplastics distribution and microbial community characteristics of farmland soil under different mulch methods

Abstract

The widespread use of plastic film in agricultural production has resulted in the accumulation of large amounts of residual plastic film in the soil, and most of the plastic residuals eventually break up into microplastics (MPs). However, the effects of different film mulching methods on the soil ecosystems are largely unexplored. Therefore, we investigated the MPs distribution and the physicochemical properties and microbial community structure in the farmland soil tillage layer covered with different mulching method of film. The results indicate that the film mulching method had no significant effect on the soil pH and organic matter content, however, the respiration intensity of the soil covered with mulching film (MF) (60.11-84.99 μg/g) and shed film (SF) (56.10-65.68 μg/g) was significantly higher than that covered with shed film & mulching film (SMF) (17.25-39.16 μg/g). The MPs abundance in the soil covered with MF (1367 particles/kg soil) was significantly higher than that covered with SF (800 particles/kg soil) and slightly higher than that with SMF (1000 particles/kg soil). The small-sized (0-0.5 mm) MPs abundance was increased with the tillage layer depth (0-20 cm), while the large-sized (1-5 mm) MPs abundance was the opposite. In addition, in the soil covered with agricultural film, the dominant phylum and genera of the bacteria were Proteobacteria (relative abundance was 64.06 %) and Pseudomonas (13.16 %), respectively. In the soil without agricultural film application as a control treatment, the diversity of the soil bacterial community was higher than that in the soil covered with agricultural film, and the relative abundances of the top 10 genera were all less than 5 %. Overall, this study provides essential information for understanding the effects of different film mulching methods on the agricultural systems. Overall, this study provides essential information for understanding the effects of different film mulching methods on the distribution of MPs and the biogeochemical properties of farmland soils.

Keywords: Agricultural film; Bacterial community structure; Microplastics; Mulching method; Soil physicochemical property; Soil tillage layer.