An alternative σ factor σL sl regulates lincomycin production in Streptomyces lincolnensis

Bingbing Tu; Yue Mao; Ruida Wang; Yajing Kang; Jiang Ye; Huizhan Zhang; Haizhen Wu

doi:10.1002/jobm.202200485

An alternative σ factor σ^L _sl regulates lincomycin production in Streptomyces lincolnensis

J Basic Microbiol. 2023 Feb;63(2):190-199. doi: 10.1002/jobm.202200485. Epub 2022 Dec 1.

Authors

Bingbing Tu¹, Yue Mao¹, Ruida Wang^{1

2}, Yajing Kang¹, Jiang Ye^{1

2}, Huizhan Zhang^{1

2}, Haizhen Wu^{1

2}

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
² Department of Applied Biology, East China University of Science and Technology, Shanghai, China.

PMID: 36453540
DOI: 10.1002/jobm.202200485

Abstract

Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative σ factor, σ^L _sl , in Streptomyces lincolnensis NRRL 2936. Deletion of sigL_sl resulted in an increase in cell growth but a decrease in lincomycin production. σ^L _sl boosted lincomycin biosynthesis by directly stimulating the transcription of four genes (lmbD, lmbV, lmrC, and lmbU) within the lincomycin biosynthetic lmb gene cluster. Besides, σ^L _sl participated in lincomycin biosynthesis by directly stimulating the transcription of mshC, a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that σ^L _sl plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.

Keywords: Streptomyces lincolnensis; alternative σ factor; lincomycin; σLsl.

MeSH terms

Anti-Bacterial Agents
Bacterial Proteins / genetics
Lincomycin*
Sigma Factor* / genetics

Substances

Lincomycin
Sigma Factor
Bacterial Proteins
Anti-Bacterial Agents

Supplementary concepts

Streptomyces lincolnensis