Nitrogen addition promotes early-stage and inhibits late-stage decomposition of fine roots in Pinus massoniana plantation

Lijun Wang; Yafei Shen; Ruimei Cheng; Wenfa Xiao; Lixiong Zeng; Pengfei Sun; Tian Chen; Meng Zhang

doi:10.3389/fpls.2022.1048153

Nitrogen addition promotes early-stage and inhibits late-stage decomposition of fine roots in Pinus massoniana plantation

Front Plant Sci. 2022 Nov 14:13:1048153. doi: 10.3389/fpls.2022.1048153. eCollection 2022.

Authors

Lijun Wang¹, Yafei Shen^{1

2}, Ruimei Cheng^{1

2}, Wenfa Xiao^{1

2}, Lixiong Zeng^{1

2}, Pengfei Sun¹, Tian Chen¹, Meng Zhang¹

Affiliations

¹ Key Laboratory of Forest Ecology and Environment, National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
² Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

Abstract

Increasing atmospheric nitrogen (N) deposition has a profound impact on the ecosystem functions and processes. Fine root decomposition is an important pathway for the reentry of nutrients into the soil. However, the effect of N addition on root decomposition and its potential mechanism is not well understood with respect to root branch orders. In this study, we conducted a 30-month decomposition experiment of fine roots under different concentrations of N addition treatments (0, 30, 60, and 90 kg N ha^-1 year^-1, respectively) in a typical Pinus massoniana plantation in the Three Gorges Reservoir Area of China. In the early stage of decomposition (0-18 months), N addition at all concentrations promoted the decomposition of fine roots, and the average decomposition rates of order 1-2, order 3-4, order 5-6 fine roots were increased by 13.54%, 6.15% and 7.96% respectively. In the late stage of decomposition (18-30 months), high N addition inhibited the decomposition of fine root, and the average decomposition rates of order 1-2, order 3-4, order 5-6 fine roots were decreased by 58.35%, 35.43% and 47.56% respectively. At the same time, N addition promoted the release of lignin, carbon (C), N, and phosphorus (P) in the early-stage, whereas high N addition inhibited the release of lignin, C, N, and the activities of lignin-degrading enzyme (peroxidase and polyphenol oxidase) in the late-stage. The decomposition constant (k) was significantly correlated with the initial chemical quality of the fine roots and lignin-degrading enzyme activities. The higher-order (order 3-4 and order 5-6) fine roots decomposed faster than lower-order (order 1-2) fine roots due to higher initial cellulose, starch, sugar, C concentrations and higher C/N, C/P, lignin/N ratios and lower N, P concentrations. In addition, low N (30 kg N ha^-1 year^-1) treatments decreased soil organic matter content, whereas high N (90 kg N ha^-1 year^-1) treatment had the opposite effect. All the N treatments reduced soil pH and total P content, indicating that increased N deposition may led to soil acidification. Our findings indicated that the effect of N addition on decomposition varied with the decomposition stages. The decomposition difference between the lower-order and higher-order fine roots were controlled strongly by the initial chemical quality of the fine roots. This study provides new insights into understanding and predicting possible changes in plant root decomposition and soil properties in the future atmospheric N deposition increase scenarios.

Keywords: decomposition; initial chemical quality; lignin degradation; nitrogen addition; root orders.