Many measures of comfort and frequency of sexual communication between partners are limited in gender/sex and sexual orientation inclusivity, how constructs are measured, and for whom. We conducted two studies to investigate a revised and extended version of the Female Partner's Communication During Sexual Activity Scale: the Sexual Communication Scale (SeCS). We revised the gender/sex language to improve inclusion and added items to assess frequency and comfort with sexual communication. In Study 1, an exploratory factor analysis (n = 578) supported a three-factor structure (Frequency of bidirectional communication, α = .96; Ease of own communication, α = .90; Ease of partner's communication, α = .83). In Study 2, a confirmatory factor analysis (n = 1479) further supported the three-factor structure. Specifically, the three-factor model provided a reasonably good fit (χ2 (44) = 511.35, p < .001, CFI = .97, GFI = .95, AGFI = .91, SRMR = .00, RMSEA = .08). In both studies, we found small or no differences in men and women's comfort and frequency of sexual communication. The results provide initial support that the SeCS is an internally consistent, multidimensional gender/sex inclusive tool for future research on sexual communication.