Structure Elucidation of Antibiotics

Julia Moschny; Georgios Daletos; Peter Proksch; Chambers C Hughes

doi:10.1007/978-1-0716-2855-3_6

Structure Elucidation of Antibiotics

Methods Mol Biol. 2023:2601:97-122. doi: 10.1007/978-1-0716-2855-3_6.

Authors

Julia Moschny¹, Georgios Daletos², Peter Proksch², Chambers C Hughes³

Affiliations

¹ Department of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany.
² Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine-University, Düsseldorf, Germany.
³ Department of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany. chambers.hughes@uni-tuebingen.de.

PMID: 36445581
DOI: 10.1007/978-1-0716-2855-3_6

Abstract

To date, there are hundreds of characterized natural products with antibacterial activity against pathogenic bacteria, and several have become bonafide antibiotic drugs. The development of antibacterial natural products into antibiotic drugs, both in the past and in the future, hinges upon an accurate description of the exact chemical structure of the compound. Bolstered by some form of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy is the primary technique for elucidating the chemical structure of organic molecules including natural products. By combining various one-dimensional (1D) and two-dimensional (2D) experiments, the connectivity between atoms is established and a complete "picture" of the molecule is thereby revealed.

Keywords: Antibiotics; HPLC-MS; NMR; Natural products; Structure elucidation; Tylosin A.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents* / pharmacology
Biological Products*
Mass Spectrometry

Substances

Anti-Bacterial Agents
Biological Products