Organic acid, phosphate, sulfate and ammonium co-metabolism releasing insoluble phosphate by Klebsiella aerogenes to simultaneously stabilize lead and cadmium

Zhihui Yang; Zixin Liu; Feiping Zhao; Lin Yu; Weichun Yang; Mengying Si; Qi Liao

doi:10.1016/j.jhazmat.2022.130378

Organic acid, phosphate, sulfate and ammonium co-metabolism releasing insoluble phosphate by Klebsiella aerogenes to simultaneously stabilize lead and cadmium

J Hazard Mater. 2023 Feb 5;443(Pt B):130378. doi: 10.1016/j.jhazmat.2022.130378. Epub 2022 Nov 10.

Authors

Zhihui Yang¹, Zixin Liu², Feiping Zhao¹, Lin Yu², Weichun Yang¹, Mengying Si¹, Qi Liao³

Affiliations

¹ Institute of Environmental Science and Engineering, School of Metallurgy and Environment, Central South University, 410083 Changsha, China; Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution, 410083 Changsha, China.
² Institute of Environmental Science and Engineering, School of Metallurgy and Environment, Central South University, 410083 Changsha, China.
³ Institute of Environmental Science and Engineering, School of Metallurgy and Environment, Central South University, 410083 Changsha, China; Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution, 410083 Changsha, China. Electronic address: liaoqi@csu.edu.cn.

PMID: 36444069
DOI: 10.1016/j.jhazmat.2022.130378

Abstract

Here, a novel phosphate-solubilizing bacterium (PSB), Klebsiella aerogenes Wn was applied to develop an environmental-friendly method to simultaneously stabilize Pb and Cd. The maximum dissolved phosphate was up to 701.36 mg/L by the strain Wn. The high performance liquid chromatography (HPLC) and Pearson correlation analyses showed that the acetic acid produced by the strain Wn was significantly positively associated with the released phosphate. Moreover, 100% of 500 mg/L of Pb and 100 mg/L of Cd were simultaneously stabilized in the classical NBRIP medium and the major products were Pb₅(PO₄)₃Cl, Ca_7.7Cd_0.8(PO₄)₈(H₂O)_2.4 and CdS, respectively. In addition, the bacterial genome and transcriptome analyses showed that the pentose phosphate pathway (PPP), pyruvate metabolism pathway, thiamine metabolic pathway, sulfate reduction and ammonium bio-transformation were coupled to promote releasing insoluble phosphate and stabilizing Pb and Cd. In the metabolism networks, the critical genes of gcd, aceE, thiE, thiS and cysH, etc. were significantly up-regulated. Our results are beneficial to deeper understand the molecular mechanisms of releasing insoluble inorganic phosphate by PSBs and develop a technology prototype to simultaneously stabilize Pb and Cd using the PSBs.

Keywords: Bacterial transcriptome; Co-metabolism; Molecular mechanism; Pb-Cd combined pollution; Phosphate-solubilizing bacteria; Stabilization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ammonium Compounds*
Cadmium
Enterobacter aerogenes*
Lead
Organic Chemicals
Phosphates
Sulfates

Substances

Sulfates
Phosphates
Cadmium
Lead
Organic Chemicals
Ammonium Compounds