Pulsed electric field (PEF) recovery of biomolecules from Chlorella: Extract efficiency, nutrient relative value, and algae morphology analysis

Min Wang; Jianjun Zhou; Juan Manuel Castagnini; Houda Berrada; Francisco J Barba

doi:10.1016/j.foodchem.2022.134615

Pulsed electric field (PEF) recovery of biomolecules from Chlorella: Extract efficiency, nutrient relative value, and algae morphology analysis

Food Chem. 2023 Mar 15;404(Pt A):134615. doi: 10.1016/j.foodchem.2022.134615. Epub 2022 Oct 17.

Authors

Min Wang¹, Jianjun Zhou¹, Juan Manuel Castagnini², Houda Berrada³, Francisco J Barba⁴

Affiliations

¹ Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, Valencia, Spain; Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
² Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, Valencia, Spain. Electronic address: juan.castagnini@uv.es.
³ Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, Valencia, Spain. Electronic address: houda.berrada@uv.es.
⁴ Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, Valencia, Spain. Electronic address: francisco.barba@uv.es.

PMID: 36444042
DOI: 10.1016/j.foodchem.2022.134615

Abstract

This study investigated the effects of pulsed electric field (PEF) (3 kV/cm, 44 pulses, 99 kJ/kg), solvent (H₂O or 50 % DMSO) and time (0, 10, 20, 30, 60, 90, 120 and 180 min) on the extraction of Chlorella antioxidant biomolecules and minerals. The results showed that PEF treatment increased the biomolecules recovery. For the extraction time of 120 min, more proteins and polyphenols were obtained using water, while more chlorophyll a and b, and carotenoids were obtained using 50 % DMSO as the extraction solvent. The extracts mineral concentration (PEF vs control) were analysed including Mg, P, Ca, Fe and Zn, and the Relative Nutrient Values results indicated that Chlorella H₂O-extracts could be used as a mineral source for different populations. Finally, the fluorescence and scanning electron microscopy revealed the electroporation effect of PEF.

Keywords: Antioxidants; Microalgae; Microstructure; Minerals; Pulsed electric fields (PEF).

MeSH terms

Chlorella*
Chlorophyll A
Dimethyl Sulfoxide
Nutrients
Nutritive Value*
Solvents

Substances

Chlorophyll A
Dimethyl Sulfoxide
Solvents