In Vitro Models for the Study of Liver Biology and Diseases: Advances and Limitations

Savneet Kaur; Srivatsan Kidambi; Martí Ortega-Ribera; Le Thi Thanh Thuy; Natalia Nieto; Victoria C Cogger; Wei-Fen Xie; Frank Tacke; Jordi Gracia-Sancho

doi:10.1016/j.jcmgh.2022.11.008

In Vitro Models for the Study of Liver Biology and Diseases: Advances and Limitations

Cell Mol Gastroenterol Hepatol. 2023;15(3):559-571. doi: 10.1016/j.jcmgh.2022.11.008. Epub 2022 Nov 26.

Authors

Savneet Kaur¹, Srivatsan Kidambi², Martí Ortega-Ribera³, Le Thi Thanh Thuy⁴, Natalia Nieto⁵, Victoria C Cogger⁶, Wei-Fen Xie⁷, Frank Tacke⁸, Jordi Gracia-Sancho⁹

Affiliations

¹ Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India.
² Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska.
³ Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts.
⁴ Department of Hepatology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan.
⁵ Department of Pathology, University of Illinois at Chicago, Chicago, Illinois.
⁶ Faculty of Medicine and Health, University of Sydney, Sydney, Australia.
⁷ Department of Gastroenterology, Changzheng Hospital, Naval Medical University, Shanghai, China.
⁸ Department of Hepatology and Gastroenterology, Charité Universitätsmedizin Berlin, Berlin, Germany.
⁹ Liver Vascular Biology, IDIBAPS Biomedical Research Institute, CIBEREHD, Barcelona, Spain; Department of Visceral Surgery and Medicine, Inselspital, Bern University Hospital, University of Bern, Switzerland; Department for BioMedical Research, Visceral Surgery and Medicine, University of Bern, Switzerland. Electronic address: jgracia@recerca.clinic.cat.

Abstract

In vitro models of liver (patho)physiology, new technologies, and experimental approaches are progressing rapidly. Based on cell lines, induced pluripotent stem cells or primary cells derived from mouse or human liver as well as whole tissue (slices), such in vitro single- and multicellular models, including complex microfluidic organ-on-a-chip systems, provide tools to functionally understand mechanisms of liver health and disease. The International Society of Hepatic Sinusoidal Research (ISHSR) commissioned this working group to review the currently available in vitro liver models and describe the advantages and disadvantages of each in the context of evaluating their use for the study of liver functionality, disease modeling, therapeutic discovery, and clinical applicability.

Keywords: Bioengineering; Cirrhosis; Hepatic Sinusoid; Mechanobiology; NAFLD; NASH; Omics.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biology*
Humans
Liver* / metabolism
Mice

Abstract

Publication types

MeSH terms

Grants and funding