Guide isolation of guaiane-type sesquiterpenoids from Daphne tangutica maxim. And their anti-inflammatory activities

Abstract

Using liquid chromatography with tandem mass spectrometry guided molecular networking, 12 undescribed guaiane-type sesquiterpenoids, namely tanguticatins A-L, 19 known analogs and a previously undescribed triterpene (tanguticatin M) were obtained from Daphne tangutica Maxim and characterized. Their planar structures and configurations were elucidated and unequivocally assigned by detailed spectroscopic analyses, electronic circular dichroism spectral calculations and single single-crustal X-ray diffraction analysis. All the isolated compounds were evaluated for lipopolysaccharide-induced nitric oxide production in murine microglial BV2 cells. Tanguticatin E and K exhibited more potent inhibitory effects than minocycline (positive control).

Keywords: Anti-inflammatory; Daphne tangutica; Guaiane sesquiterpenoids; Molecular networking; Thymelaeaceae.