MicroRNA Profiling of Cell Lines and Xenografts by Quantitative PCR : MicroRNA Expression Level Determination by qPCR

Ariadna Boloix; Miguel F Segura

doi:10.1007/978-1-0716-2823-2_7

MicroRNA Profiling of Cell Lines and Xenografts by Quantitative PCR : MicroRNA Expression Level Determination by qPCR

Methods Mol Biol. 2023:2595:101-114. doi: 10.1007/978-1-0716-2823-2_7.

Authors

Ariadna Boloix¹, Miguel F Segura²

Affiliations

¹ Group of Childhood Cancer and Blood Disorders, Hospital Universitari Vall d'Hebron, Vall d'Hebron Institut de Recerca, VHIR, Universitat Autònoma de Barcelona, Barcelona, Spain.
² Group of Childhood Cancer and Blood Disorders, Hospital Universitari Vall d'Hebron, Vall d'Hebron Institut de Recerca, VHIR, Universitat Autònoma de Barcelona, Barcelona, Spain. miguel.segura@vhir.org.

PMID: 36441457
DOI: 10.1007/978-1-0716-2823-2_7

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that play essential roles in regulating gene expression at the post-transcriptional level, possibly at any level of the cellular physiology. Furthermore, their deregulation has been observed in a myriad of human diseases including cancer. Therefore, miRNA-based therapies are directed to inhibit the function of oncogenic miRNA or to restore the function of tumor-suppressive miRNAs. Here, we describe how to analyze miRNA levels after the transfection of miRNAs of interest using different transfection reagents or intravenous administration of miRNAs conjugated to lipid nanoparticles in cell lines and in mouse xenograft models.

Keywords: Cancer; Real-time quantitative PCR (qPCR); miRNA-based therapies; microRNA.

MeSH terms

Animals
Cell Line
Disease Models, Animal
Heterografts
Humans
Mice
MicroRNAs* / genetics
Real-Time Polymerase Chain Reaction

Substances

Lipid Nanoparticles
MicroRNAs