Detection of MicroRNAs in Brain Slices Using In Situ Hybridization

Sean Quinlan; Christine Henke; Gary P Brennan; David C Henshall; Eva M Jimenez-Mateos

doi:10.1007/978-1-0716-2823-2_6

Detection of MicroRNAs in Brain Slices Using In Situ Hybridization

Methods Mol Biol. 2023:2595:93-100. doi: 10.1007/978-1-0716-2823-2_6.

Authors

Sean Quinlan¹, Christine Henke¹, Gary P Brennan¹, David C Henshall¹, Eva M Jimenez-Mateos²

Affiliations

¹ Discipline of Physiology, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.
² Discipline of Physiology, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland. JIMENEZE@tcd.ie.

PMID: 36441456
DOI: 10.1007/978-1-0716-2823-2_6

Abstract

MicroRNAs are key posttranscriptional regulators of protein levels in cells. The brain is particularly enriched in microRNAs, and important roles have been demonstrated for these noncoding RNAs in various neurological disorders. To this end, visualization of microRNAs in specific cell types and subcellular compartments within tissue sections provides researchers with essential insights that support understanding of the cell and molecular mechanisms of microRNAs in brain diseases. In this chapter we describe an in situ hybridization protocol for the detection of microRNAs in mouse brain sections, which provides cellular resolution of the expression of microRNAs in the brain.

Keywords: Brain; CNS; In situ hybridization; Neurodegenerative disorders; Staining; microRNA.

MeSH terms

Animals
Brain
Brain Diseases*
Humans
In Situ Hybridization
Mice
MicroRNAs* / genetics
Research Personnel

Substances

MicroRNAs