Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate

Na Han; Wen-Ji Geng; Jian Li; Shu-Ting Liu; Jie Zhang; Yi-Jie Wen; Huai-Hua Xu; Meng-Yuan Li; Yan-Ru Li; Pei-Pei Han

doi:10.3389/fpls.2022.1040596

Transcription level differences in Taxus wallichiana var. mairei elicited by Ce³⁺, Ce⁴⁺ and methyl jasmonate

Front Plant Sci. 2022 Nov 10:13:1040596. doi: 10.3389/fpls.2022.1040596. eCollection 2022.

Authors

Na Han¹, Wen-Ji Geng¹, Jian Li¹, Shu-Ting Liu¹, Jie Zhang¹, Yi-Jie Wen¹, Huai-Hua Xu¹, Meng-Yuan Li¹, Yan-Ru Li¹, Pei-Pei Han¹

Affiliation

¹ Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.

Abstract

Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce³⁺, Ce⁴⁺ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce³⁺ and 1 mM Ce⁴⁺. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.

Keywords: MJ; T. mairei; cerium; taxol biosynthesis; transcriptome.