Evaluation of the pathogenesis of non-typical strain with α-hemolysin, Vibrio parahaemolyticus 353, isolated from Chinese seafood through comparative genome and transcriptome analysis

Fei Zha; Rui Pang; Shixuan Huang; Jumei Zhang; Juan Wang; Moutong Chen; Liang Xue; Qinghua Ye; Shi Wu; Meiyan Yang; Qihui Gu; Yu Ding; Hao Zhang; Qingping Wu

doi:10.1016/j.marpolbul.2022.114276

Evaluation of the pathogenesis of non-typical strain with α-hemolysin, Vibrio parahaemolyticus 353, isolated from Chinese seafood through comparative genome and transcriptome analysis

Mar Pollut Bull. 2023 Jan:186:114276. doi: 10.1016/j.marpolbul.2022.114276. Epub 2022 Nov 24.

Authors

Fei Zha¹, Rui Pang², Shixuan Huang², Jumei Zhang², Juan Wang², Moutong Chen², Liang Xue², Qinghua Ye², Shi Wu², Meiyan Yang², Qihui Gu², Yu Ding³, Hao Zhang⁴, Qingping Wu⁵

Affiliations

¹ School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China; Guangdong Provincial Key Laboratory of Microbial Safety and Health, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Microbiomics and Precision Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, China.
² Guangdong Provincial Key Laboratory of Microbial Safety and Health, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Microbiomics and Precision Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, China.
³ Department of Food Science and Engineering, Institute of Food Safety & Nutrition, Jinan University, Guangzhou 510632, Guangdong, China.
⁴ School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: zhanghao@jiangnan.edu.cn.
⁵ Guangdong Provincial Key Laboratory of Microbial Safety and Health, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Microbiomics and Precision Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, China. Electronic address: wuqp203@163.com.

PMID: 36437125
DOI: 10.1016/j.marpolbul.2022.114276

Abstract

Vibrio parahaemolyticus outbreaks frequently occur, causing gastrointestinal sickness owing to the consumption of aquatic foods by various virulence factors; however, the mechanism of pathogenesis is still unknown. In this study, a non-typical strain of V. parahaemolyticus, named VP353, was isolated from shrimp in China. Its comparative genome and transcriptome after infection with Caco-2 cells were examined to illustrate the mechanisms of its pathogenesis. VP353 was a tdh-trh- strain but uncommonly manifested robust cytotoxicity towards Caco-2 cells. Compared with the standard strain RIMD2210633, VP353 harbored alpha-hemolysins (hlyA, hlyB, hlyC, and hlyD) was first reported in V. parahaemolyticus and showed high diversity in the T3SS2 gene cluster. Moreover, the expression of flagella, T2SS, quorum sensing-related genes, hlyA, hlyC were up-regulated, and hlyB, hlyD were down-regulated. In summary, our results demonstrate that some novel virulence factors contribute to the pathogenesis of V. parahaemolyticus infection.

Keywords: Aquatic food; Caco-2 cells; Comparative genome; Genome-sequencing; Transcriptome; Virulence factors.

MeSH terms

Caco-2 Cells
Gene Expression Profiling
Hemolysin Proteins / genetics
Humans
Seafood / analysis
Vibrio Infections*
Vibrio parahaemolyticus*
Virulence Factors / genetics

Substances

Hemolysin Proteins
Virulence Factors