Microcapsules based on alginate and guar gum for co-delivery of hydrophobic antitumor bioactives

Louhana M Rebouças; Alexandre C C Sousa; Caroline G Sampaio; Larissa M R Silva; Pedro M S Costa; Cláudia Pessoa; Nilce V G P S Brasil; Nágila M P S Ricardo

doi:10.1016/j.carbpol.2022.120310

Microcapsules based on alginate and guar gum for co-delivery of hydrophobic antitumor bioactives

Carbohydr Polym. 2023 Feb 1;301(Pt A):120310. doi: 10.1016/j.carbpol.2022.120310. Epub 2022 Nov 8.

Authors

Louhana M Rebouças¹, Alexandre C C Sousa², Caroline G Sampaio³, Larissa M R Silva⁴, Pedro M S Costa⁵, Cláudia Pessoa⁶, Nilce V G P S Brasil⁷, Nágila M P S Ricardo⁸

Affiliations

¹ Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, CE 60440-900, Brazil; Federal Institute of Education, Science and Technology of Ceará, Fortaleza, CE 60410-426, Brazil. Electronic address: louhanar@gmail.com.
² Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, CE 60440-900, Brazil; Federal Institute of Education, Science and Technology of Ceará, Fortaleza, CE 60410-426, Brazil. Electronic address: alexandre.carreira@ifce.edu.br.
³ Federal Institute of Education, Science and Technology of Ceará, Fortaleza, CE 60410-426, Brazil. Electronic address: carolinesampaio@ifce.edu.br.
⁴ Department of Food Engineering, Federal University of Ceará, Fortaleza, CE 60356-000, Brazil. Electronic address: larissamrs@ufc.br.
⁵ Laboratory of Experimental Oncology, Center for Research and Drug Development, Federal University of Ceará, Fortaleza, CE 60430-275, Brazil. Electronic address: pmikaelcosta@alu.ufc.br.
⁶ Laboratory of Experimental Oncology, Center for Research and Drug Development, Federal University of Ceará, Fortaleza, CE 60430-275, Brazil. Electronic address: cpessoa@ufc.br.
⁷ Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, CE 60440-900, Brazil. Electronic address: nilce@dqoi.ufc.br.
⁸ Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, CE 60440-900, Brazil. Electronic address: naricard@ufc.br.

PMID: 36436871
DOI: 10.1016/j.carbpol.2022.120310

Abstract

The main goal was the development of a polysaccharide microcapsule for anticancer application based on guar gum and sodium alginate for the controlled release of hesperidin and betulinic acid by spray drying technique. The microcapsule showed an Encapsulation Efficiency of 98.15 ± 0.34 % for hesperidin and 99.76 ± 0.22 % for betulinic acid. In the release study, the Korsmeyer-Peppas mathematical model was identified as the most adequate to explain the observed release mechanism. In vivo tests were performed in zebrafish model, revealing that the microcapsules did not alter the locomotor activity and were not toxic within 96 h by oral administration, suggesting their biological safety. In vitro cytotoxic activity against HL-60 cells confirmed an IC₅₀ value of 2.52 ± 0.23 μg mL^-1 in 72 h. Additionally, a decrease in the cytotoxic activity of betulinic acid against L-929 (non-tumor) cells was evidenced. Therefore, the microcapsules synthesized in this work represent a promising formulation for anticancer applications.

Keywords: Alginate; Betulinic acid; Guar gum; HL-60; Hesperidin; Polysaccharide microcapsules.

MeSH terms

Alginates*
Animals
Betulinic Acid
Capsules
Hesperidin*
Zebrafish

Substances

guar gum
Capsules
Alginates
Hesperidin
Betulinic Acid