Combined effects of vitamin C and cold atmospheric plasma-conditioned media against glioblastoma via hydrogen peroxide

Huidan Yu; Xueyan Song; Fan Yang; Jun Wang; Mingjian Sun; Guangxin Liu; Nafees Ahmad; Yuanshuai Zhou; Yina Zhang; Guohua Shi; Ruobing Zhang; Jianping Liu; Xiaobing Jiang; Peng Fu; Gang Chen; Jingmei Li; Jie Zhuang; Minxuan Sun

doi:10.1016/j.freeradbiomed.2022.11.028

Combined effects of vitamin C and cold atmospheric plasma-conditioned media against glioblastoma via hydrogen peroxide

Free Radic Biol Med. 2023 Jan:194:1-11. doi: 10.1016/j.freeradbiomed.2022.11.028. Epub 2022 Nov 24.

Authors

Huidan Yu¹, Xueyan Song², Fan Yang³, Jun Wang³, Mingjian Sun⁴, Guangxin Liu³, Nafees Ahmad⁵, Yuanshuai Zhou³, Yina Zhang⁶, Guohua Shi³, Ruobing Zhang³, Jianping Liu⁷, Xiaobing Jiang⁸, Peng Fu⁸, Gang Chen⁹, Jingmei Li¹⁰, Jie Zhuang¹¹, Minxuan Sun¹²

Affiliations

¹ School of Life Sciences, Changchun University of Science and Technology, Changchun, 130022, China; Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China. Electronic address: zyx200128@163.com.
² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China. Electronic address: songxy@sibet.ac.cn.
³ Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
⁴ Measurement and Control Research Center Department of Control Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China.
⁵ Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
⁶ Neurological Department, Helios-Amper Clinic, Dachau, Germany.
⁷ Integrated Cardio Metabolic Centre, Karolinska Institute, Huddinge, Sweden.
⁸ Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁹ Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.
¹⁰ School of Life Sciences, Changchun University of Science and Technology, Changchun, 130022, China. Electronic address: lijm@cust.edu.cn.
¹¹ Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China. Electronic address: Zhuangj@sibet.ac.cn.
¹² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China. Electronic address: minxuan.sun@sibet.ac.cn.

PMID: 36436726
DOI: 10.1016/j.freeradbiomed.2022.11.028

Abstract

Glioblastoma is the most lethal intracranial malignant tumor, for which the five-year overall survival rate is approximately 5%. Here we explored the therapeutic combination of vitamin C and plasma-conditioned medium on glioblastoma cells in culture and as subcutaneous or intracranial xenografts in mice. The combination treatment reduced cell viability and proliferation while promoting apoptosis, and the effects were significantly stronger than with either treatment on its own. Similar results were obtained in the two xenograft models. Vitamin C appeared to upregulate aquaporin-3 and enhance the uptake of extracellular H₂O₂, while the combination treatment increased intracellular levels of reactive oxygen species including H₂O₂ and activated the JNK signaling pathway. The cytotoxic effects of the combination treatment were partially reversed by the specific JNK signaling inhibitor SP600125. Our results suggest that the combination of vitamin C and plasma-conditioned medium has therapeutic potential against glioblastoma, and they provide mechanistic insights that may help investigate this and other potential therapies in greater depth.

Keywords: AQP3; Glioblastoma cells; Plasma-conditioned medium; ROS; Vitamin C.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents* / pharmacology
Apoptosis
Ascorbic Acid / pharmacology
Cell Line, Tumor
Culture Media, Conditioned / pharmacology
Glioblastoma* / metabolism
Humans
Hydrogen Peroxide / metabolism
Mice
Reactive Oxygen Species / metabolism
Vitamins / pharmacology

Substances

Hydrogen Peroxide
Culture Media, Conditioned
Ascorbic Acid
Antineoplastic Agents
Reactive Oxygen Species
Vitamins