Response surface methodology optimization and HPLC-ESI-QTOF-MS/MS analysis on ultrasonic-assisted extraction of phenolic compounds from okra (Abelmoschus esculentus) and their antioxidant activity

Abstract

Okra (Abelmoschus esculentus) has attracted a growing attention for its nutritional and medicinal values, while few studies focused on systemic study of okra polyphenols (OP). In order to obtain the maximum extracted efficiency, response surface methodology was used to optimize ultrasonic-assisted extraction conditions. The maximum TPC was 7.02 mg GAE/g dw under the condition of solid-liquid ratio 1:25, ethanol concentration 70 %, 40 min, and 142 W at 46 °C. Then 27 compounds in OP were identified by HPLC-ESI-QTOF-MS/MS, among which 7-hydroxycoumarin, scopoletin, luteolin and et al were firstly identified from okra. Furthermore, OP exhibited antioxidant activity in reducing power (FRAP, 9.77 mM Fe²⁺/g OP) and radical scavenging (DPPH, IC₅₀ 19.31 µg/mL; SARC, IC₅₀ 210.81 µg/ml). Moreover, OP significantly inhibited cell apoptosis and ROS generation, and alleviated oxidative damage in t-BHP induced HUVECs. Overall, our findings could provide perspective for further potential employments of okra as functional food.

Keywords: Antioxidant activity; HPLC-ESI-QTOF-MS/MS; Okra polyphenols; Response surface methodology; Ultrasonic-assisted extraction.