Enhanced nitrogen removal from low C/N municipal wastewater employing algal biochar supported nano zero-valent iron (ABC-nZVI) using A/A/O-MBR: Duration and rehabilitation

Abstract

To bridge the organic-dependent barrier on nitrogen from low carbon/nitrogen (C/N) municipal wastewater, employing algal biochar supported nano zero-valent iron (ABC-nZVI) was investigated using A/A/O-MBR. Firstly, it can be seen that adequate carbon source is indispensable for the removal, since total nitrogen (TN) removal reached 77.89 % with the influent C/N of 7.8. Secondly, conducted in batch experiments with different doses of ABC-nZVI with/without active sludge, removal efficiency of total inorganic nitrogen (TIN) and the effective time achieved 84.94 % and 24 h with an ABC-nZVI dose of 300 mg/L, respectively. Thirdly, it was found that the duration of high-efficiency denitrification reached 9 h with the addition of 250 mg/L of ABC-nZVI to the anoxic tank of A/A/O-MBR, and the effluent ammonium nitrogen (NH₄⁺-N) also meet the national discharge standard. Besides, biodiversity of both anoxic and aerobic sludge was apparently promoted with the addition of ABC-nZVI, while the lab-scale A/A/O-MBR could also be fully rehabilitated within 12 h. Finally, predicted through PICRUSt2, relevant abundance of functional genes involved in nitrogen metabolism could be enriched by nZVI addition. As an alternative supporting electron donor and mediator, ABC-nZVI can also be participated in the enhanced nitrogen removal in A/A/O-MBR at low C/N.

Keywords: Algal biochar-nZVI; Anaerobic/anoxic/aerobic-MBR; Low C/N ratio; Nitrogen removal; PICRUSt2.