A sensitive, accurate, and rapid multimode dot-filtration immunoassay (MDFIA) was established for the detection of Salmonella typhimurium using the intrinsic color, catalytic property, and photothermal effect of magnetic molybdenum disulphide (MoS2@Fe3O4). The critical performance parameters of MDFIA were optimized in detail. The sensitivity of MDFIA can be improved by the catalytic color development and photothermal conversion of MoS2@Fe3O4 with a limit of detection (LOD) of 101 CFU·mL-1, which is an order of magnitude lower than direct visual detection (102 CFU·mL-1). Besides, the magnetic property of MoS2@Fe3O4 was used for the rapid enrichment and separation of the target allowing detection of trace concentrations of Salmonella typhimurium. The selectivity and applicability of the MDFIA were verified in spiked samples, indicating that the established assay may have bright application prospects for the detection and control of foodborne pathogens. A multimode dot-filtration immunoassay was constructed for Salmonella typhimurium rapid detection based on the peroxidase-like activity, magnetic property, and photothermal effect of MoS2@Fe3O4.
Keywords: Catalysis; Colorimetry; MoS2@Fe3O4; Multimode dot-filtration immunoassay; Photothermal effect; Salmonella typhimurium.
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.