Electrochemical Gene Amplification Signal Detection of Disposable Biochips Using Electrodes

Sensors (Basel). 2022 Nov 9;22(22):8624. doi: 10.3390/s22228624.

Abstract

Real-time Polymerase Chain Reaction (RT-PCR), a molecular diagnostic technology, is spotlighted as one of the quickest and fastest diagnostic methods for the actual coronavirus (SARS-CoV-2). However, the fluorescent label-based technology of the RT-PCR technique requires expensive equipment and a sample pretreatment process for analysis. Therefore, this paper proposes a biochip based on Electrochemical Impedance Spectroscopy (EIS). In this paper, it was possible to see the change according to the concentration by measuring the impedance with a chip made of two electrodes with different shapes of sample DNA.

Keywords: PCR chip; biochip; impedance spectroscopy; label-free detection; point of care testing (POCT).

MeSH terms

  • COVID-19* / diagnosis
  • Electrodes
  • Gene Amplification*
  • Humans
  • RNA, Viral / analysis
  • SARS-CoV-2 / genetics

Substances

  • RNA, Viral