Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease

Mike R Milward; Martin R Ling; Melissa M Grant; Joanna Batt; Iain L C Chapple

doi:10.1007/978-1-0716-2780-8_21

Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease

Methods Mol Biol. 2023:2588:371-392. doi: 10.1007/978-1-0716-2780-8_21.

Authors

Mike R Milward¹, Martin R Ling², Melissa M Grant¹, Joanna Batt¹, Iain L C Chapple³

Affiliations

¹ The School of Dentistry, University of Birmingham, Birmingham, UK.
² Oral Heath R&D, GSK, Weybridge, UK.
³ The School of Dentistry, University of Birmingham, Birmingham, UK. i.l.c.chapple@bham.ac.uk.

PMID: 36418698
DOI: 10.1007/978-1-0716-2780-8_21

Abstract

The interactions between bacteria, epithelium, and neutrophilic polymorphonuclear leukocytes (neutrophils) are the key to the initiation and progression of many chronic inflammatory-immune diseases. In addition, all can be influenced by external factors, such as micronutrients, thereby providing potentially novel approaches to therapy. This chapter will therefore provide detailed methods for core techniques involved in studying cellular and molecular epithelial responses to a bacterial challenge in relation to chronic inflammatory disease pathogenesis and therapy.

Keywords: DNA; Electrophoresis; Epithelium; Gene expression; H400 oral epithelial cells; Immunocytochemistry; NF-kappa B; Polymerase chain reaction; RNA.

MeSH terms

Cell Culture Techniques*
Chronic Disease
Epithelial Cells*
Epithelium
Humans
Immunologic Tests
Research