OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development

Gerelchimeg Bou; Jia Guo; Shichao Liu; Shimeng Guo; Gantulga Davaakhuu; Qi Lv; Binghua Xue; Shupei Qiao; Jiawei Lv; Xiaogang Weng; Jianchao Zhao; Yuting Zhang; Yilong He; Heng Zhang; Zhuang Chai; Yan Liu; Yanbo Yu; Bo Qu; Ruizhen Sun; Xinghui Shen; Lei Lei; Zhonghua Liu

doi:10.1016/j.celrep.2022.111644

OCT4 expression transactivated by GATA protein is essential for non-rodent trophectoderm early development

Cell Rep. 2022 Nov 22;41(8):111644. doi: 10.1016/j.celrep.2022.111644.

Authors

Gerelchimeg Bou¹, Jia Guo², Shichao Liu², Shimeng Guo², Gantulga Davaakhuu³, Qi Lv⁴, Binghua Xue², Shupei Qiao⁵, Jiawei Lv², Xiaogang Weng², Jianchao Zhao², Yuting Zhang², Yilong He², Heng Zhang², Zhuang Chai², Yan Liu², Yanbo Yu⁶, Bo Qu⁶, Ruizhen Sun⁷, Xinghui Shen⁷, Lei Lei⁷, Zhonghua Liu⁸

Affiliations

¹ College of Life Science, Northeast Agricultural University, Harbin 150030, China; College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
² College of Life Science, Northeast Agricultural University, Harbin 150030, China.
³ Institute of Biology, Mongolian Academy of Science, Ulaanbaatar 13330, Mongolia.
⁴ College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
⁵ Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China.
⁶ Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin 150030, China.
⁷ Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China.
⁸ College of Life Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: liuzhonghua@neau.edu.cn.

PMID: 36417852
DOI: 10.1016/j.celrep.2022.111644

Abstract

Oct4 is exclusively expressed in rodent inner cell mass (ICM) but silenced in its trophectoderm (TE). However, for many non-rodent animals, including pig, cattle, rabbit, goat, and human, OCT4 has a remarkable expression in early TE. This study, applying pig as the main research model, proves that OCT4 expression in TE is supported by a unique GATA motif in the OCT4 upstream conserved regulatory region, and GATA4 is responsible for its activation. Moreover, OCT4 acts as a specific regulator of a narrow range of genes (including BCL2A1 and HNRNP2AB1) that are essential for the first wave of rapid proliferation in early TE. This study describes the regulatory mechanism to direct the OCT4 expression and its significance in TE of porcine preimplantation embryo.

Keywords: CDX2; CP: Developmental biology; CP: Molecular biology; GATA4; OCT4; early trophectoderm; molecular regulation; pig; rapid proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blastocyst*
Cattle
Humans
Rabbits
Rodentia*
Swine