Nesfatin-1, Dopamine, and NADPH levels in Infertile Women with Polycystic Ovary Syndrome: Is There a Relationship Between Their Levels and Metabolic and Hormonal Variables

Enas Ahmed Hamed; Hayam Gaber Sayyed; Ahmed Mohamed Abbas; Mohamed Maher Abdel Gaber; Hassnaa Mahmoud Abd El Aleem

doi:10.18502/jri.v23i3.10006

Nesfatin-1, Dopamine, and NADPH levels in Infertile Women with Polycystic Ovary Syndrome: Is There a Relationship Between Their Levels and Metabolic and Hormonal Variables

J Reprod Infertil. 2022 Jul-Sep;23(3):160-168. doi: 10.18502/jri.v23i3.10006.

Authors

Enas Ahmed Hamed¹, Hayam Gaber Sayyed¹, Ahmed Mohamed Abbas², Mohamed Maher Abdel Gaber³, Hassnaa Mahmoud Abd El Aleem¹

Affiliations

¹ Department of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt.
² Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, Egypt.
³ Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Abstract

Background: Polycystic ovary syndrome (PCOS) is commonest endocrine disease occurring in women of reproductive age. This study conducted to clarify altered concentrations of Nesfatin-1, nicotinamide adenine dinucleotide phosphate (NADPH), and dopamine in PCOS women and controls. Also, to assess their role in PCOS pathophysiology and their correlation with measured biochemical parameters.

Methods: In this observational study, 60 PCOS patients and 24 controls included. Medical history was recorded and full examinations were done. Serum concentrations of lipid profile, fasting blood glucose (FBG), fasting insulin (FSI), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, testosterone, progesterone, estradiol, Nesfatin-1, dopamine, and NADPH were measured by ELISA kits. Values were analyzed using unpaired t-test and Pearson Chi-square test. The p<0.05 was considered statistically significant.

Results: In this study, there was significantly elevated waist hip ratio (WHR) and body mass index (BMI) in PCOS patients versus controls (p<0.0001 and p=0.014). There was significant increase in FSH, LH, prolactin, estradiol, testosterone, Nesfatin-1, and dopamine (p=0.021, p=0.015, p<0.0001, p<0.0001, p=0.006, p=0.017, p< 0.0001) and decrease of NADPH (p<0.0001) in PCOS patients. There were significant positive correlations between Nesfatin-1, prolactin, and dopamine levels. Also, there was significant positive correlation between dopamine and BMI, FSI, FSH, LH, estradiol, and prolactin levels; however, significant negative correlations observed between NADPH and BMI, FSI, estradiol, and prolactin levels.

Conclusion: Elevated serum Nesfatin-1 concentrations and their association with hyperprolactinemia indicate that they have a role in PCOS pathophysiology. Moreover, elevated dopamine and decreased NADPH concentrations could play role in PCOS pathogenesis.

Keywords: Dopamine; Infertility; Nesfatin-1; Nicotinamide adenine dinucleotide phosphate; Polycystic ovary syndrome; Prolactin.