Relation between redox potential and natural antibody levels in goat kid serum

Abstract

The redox potential and the natural antibody (NAb) levels are parameters commonly tested to verify the physiological response to stressful situations determined by farming conditions or research needs. In this paper, the redox potential, assessed by different spectrophotometric methods, was related to NAb levels together with total immunoglobulin (tot-Ig) levels in goat kid serum. Reactive oxygen metabolites (ROMs) and ceruloplasmin (CP) oxidase activity were significantly associated with NAb-IgG and NAb-IgM. Nitric oxide metabolites (NOx) and total thiol levels (TTLs) were significantly related to NAb-IgM and tot-IgM, and with NAb-IgG, respectively. A tendency was shown between ROMs and tot-IgM, and between CP and tot-IgM, where, however, the significance levels were above the cut-off values. Total oxidant status (TOS), total antioxidant activity (TAA), determined by the ABTS-based method, the ferric reducing ability of plasma (FRAP), and free radical scavenging activity (FRSA) were associated with neither NAb nor tot-Ig. The obtained results are discussed in light of the possible linkage between the (anti)oxidant status and the innate immune system in goats.

Keywords: (Anti)oxidant status; Goat; Health biomarkers; Natural antibodies; Redox potential; Total immunoglobulins.