Transcriptome reveals the exposure effects of CeO2 nanoparticles on pakchoi (Brassica chinensis L.) photosynthesis

Jie Hong; Siying Jia; Chao Wang; Yi Li; Feng He; Jorge L Gardea-Torresdey

doi:10.1016/j.jhazmat.2022.130427

Transcriptome reveals the exposure effects of CeO₂ nanoparticles on pakchoi (Brassica chinensis L.) photosynthesis

J Hazard Mater. 2023 Feb 15;444(Pt A):130427. doi: 10.1016/j.jhazmat.2022.130427. Epub 2022 Nov 17.

Authors

Jie Hong¹, Siying Jia², Chao Wang², Yi Li³, Feng He², Jorge L Gardea-Torresdey⁴

Affiliations

¹ College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China. Electronic address: jhong@zjut.edu.cn.
² College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.
³ College of Life Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
⁴ Department of Chemistry and Biochemistry, The University of Texas, El Paso, TX 79968, United States.

PMID: 36410248
DOI: 10.1016/j.jhazmat.2022.130427

Abstract

In this study, soil-grown pakchoi after 2 weeks seedling cultivation were exposed to CeO₂ nanoparticles (CeO₂ NPs) at 0.7, 7, 70, and 350 mg kg^-1 for 30 days. Results showed that chlorophyll content and photosynthetic assimilation rate were decreased significantly under all treatments with the largest decrease of 34.16% (0.7 mg kg^-1 CeO₂ NPs), however, sub-stomatal CO₂ was increased dramatically under low dose of CeO₂ NPs (0.7 mg kg^-1). There were 4576, 3548, 2787, and 2514 genes up/down regulated significantly by 0.7, 7, 70, and 350 mg kg^-1 CeO₂ NPs, respectively, and 767 genes affected under all treatments. In addition, 0.7 mg kg^-1 CeO₂ NPs up-regulated 10 chlorophyll synthesis genes, 20 photosynthesis genes, and 10 carbon fixation enzyme genes; while 350 mg kg^-1 CeO₂ NPs down-regulated 5 photosynthesis genes and 28 auxin-activated genes. Among the key genes of photosynthesis, Ferredoxin-NADP reductase (PetH) was upregulated in 0.7, 7 and 70 mg kg^-1 treatments, while Photosystem II lipoprotein (Psb27) was downregulated under 7, 70 and 350 mg kg^-1 treatments. Top 20 metabolic pathways affected by CeO₂ NPs including plant hormone, amino acids, and glutathione, and carbon metabolism These results provide information about utilizing CeO₂ NPs more safely and effectively in the future.

Keywords: CeO(2) NPs; Gene expression; Pakchoi; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brassica*
Chlorophyll
Nanoparticles* / toxicity
Photosynthesis
Transcriptome

Substances

Chlorophyll