Fully integrated colorimetric sensor based on transparency substrate for salbutamol determination

Atchara Lomae; Sudkate Chaiyo; Orawon Chailapakul; Weena Siangproh; Janjira Panchompoo

doi:10.1016/j.mex.2022.101913

Fully integrated colorimetric sensor based on transparency substrate for salbutamol determination

MethodsX. 2022 Nov 5:9:101913. doi: 10.1016/j.mex.2022.101913. eCollection 2022.

Authors

Atchara Lomae¹, Sudkate Chaiyo², Orawon Chailapakul¹, Weena Siangproh³, Janjira Panchompoo¹

Affiliations

¹ Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
² The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
³ Department of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand.

Abstract

A facile colorimetric method based on a typical redox reaction was first developed for the determination of salbutamol (SAL) using a low-cost and portable transparency-based analytical device (TAD). The TAD was simply fabricated by wax-printing onto a transparent polymer-based substrate to create the hydrophobic barriers and the colorimetric reaction zones where the color changes could be easily observed with the naked eye. Potassium permanganate (KMnO₄), a common oxidizing agent, was deliberately used as a colorimetric reagent for SAL. Once SAL reacted with KMnO₄ in the acidified system, it could undergo oxidation and the color of KMnO₄ subsequently changed from light pink to orange. The color change corresponding to the SAL concentration could be clearly observed at the TAD sensor. In addition, the reaction color could be recorded using a digital camera and then analyzed by ImageJ for quantitative analysis. Under the optimized conditions, the developed method together with the TAD sensor exhibited high efficiency for SAL determination with linearity ranging from 0.5 to 40 mg·L^-1 and a limit of detection (LOD) of 0.05 mg·L^-1. •This proposed TAD-based colorimetric method using permanganate as color reagent showed excellent performance in SAL detection with good accuracy and high precision.

Keywords: Colorimetric detection; Potassium permanganate; Redox reaction; Salbutamol; Transparency-based analytical device.