Generation of induced pluripotent stem cells (cmESF-iPS-C5) derived from cynomolgus monkey ear skin fibroblasts (cmESF)

Xing Zhen; Sang-Je Park; Se-Hee Choe; Dong-Seok Lee; Jong-Hee Lee

doi:10.1016/j.scr.2022.102977

Generation of induced pluripotent stem cells (cmESF-iPS-C5) derived from cynomolgus monkey ear skin fibroblasts (cmESF)

Stem Cell Res. 2022 Dec:65:102977. doi: 10.1016/j.scr.2022.102977. Epub 2022 Nov 15.

Authors

Xing Zhen¹, Sang-Je Park², Se-Hee Choe², Dong-Seok Lee³, Jong-Hee Lee⁴

Affiliations

¹ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Nanoscience and Nanotechnology, Graduate School, Kyungpook National University, Daegu, Republic of Korea.
² National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea.
³ Department of Nanoscience and Nanotechnology, Graduate School, Kyungpook National University, Daegu, Republic of Korea; School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu, Republic of Korea. Electronic address: lee1@knu.ac.kr.
⁴ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology (UST), Daejeon, Republic of Korea. Electronic address: jonglee@kribb.re.kr.

PMID: 36403550
DOI: 10.1016/j.scr.2022.102977

Abstract

Cynomolgus monkeys, due to their close anatomical, genetic and physiological similarity to humans, have been employed as a popular laboratory non-human primate model over rodents. Primate animal induced pluripotent stem cell (iPSC) have been used to aid on the investigation of autologous regenerative therapies. Here, we reprogrammed cynomolgus monkey ear skin fibroblasts (cmESFs) into iPSCs as a starting material for autologous based study. The resulting cmESF-iPSCs with canonical features of PSCs will advance the development of autologous transplantation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Induced Pluripotent Stem Cells*
Macaca fascicularis