Generation of a NIS-EGFP-Fluc triple-reporter human embryonic stem cell line by PiggyBac transposon system

Pufeng Huang; Chuanhuizi Tian; Yanyan Chen; Meng Zhou; Jie Ni; Mengda Niu; Xujie Liu; Zhiping Peng

doi:10.1016/j.scr.2022.102968

Generation of a NIS-EGFP-Fluc triple-reporter human embryonic stem cell line by PiggyBac transposon system

Stem Cell Res. 2022 Dec:65:102968. doi: 10.1016/j.scr.2022.102968. Epub 2022 Nov 14.

Authors

Pufeng Huang¹, Chuanhuizi Tian², Yanyan Chen², Meng Zhou³, Jie Ni³, Mengda Niu², Xujie Liu⁴, Zhiping Peng⁵

Affiliations

¹ Department of Radiological Medicine and Oncology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China; Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, Shenzhen 518057, Guangdong Province, China.
² Department of Radiological Medicine and Oncology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.
³ Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, Shenzhen 518057, Guangdong Province, China.
⁴ Shenzhen Key Laboratory of Cardiovascular Disease, Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, Shenzhen 518057, Guangdong Province, China. Electronic address: jason.liu.xujie@gmail.com.
⁵ Department of Radiological Medicine and Oncology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China. Electronic address: pengzhiping@cqmu.edu.cn.

PMID: 36403548
DOI: 10.1016/j.scr.2022.102968

Abstract

One major challenge in stem cell therapy is to longitudinally track cell fate after cells transplantation. Molecular Imaging approaches enabling noninvasive long-term monitoring the transplanted cells are imperative for assessment of the safety and efficiency. Here, we used PiggyBac technology to insert triple reporter genes: NIS, EGFP and Firefly luciferase into a human embryonic stem cell line (hESCs, H9) and obtained a reporter hESCs line (NIS-EGFP-Fluc H9). The triple-reporters allows the transplanted NIS-EGFP-Fluc H9 cells and their derivates to be fluorescence, bioluminescence and even PET/SPECT imaged. This triple-reporter hESCs line provides a valuable imaging platform for cell-based therapeutics clinical translation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Human Embryonic Stem Cells*
Humans