A glutamate receptor-like gene is involved in ABA-mediated growth control in Physcomitrium (Physcomitrella) patens

Plant Signal Behav. 2022 Dec 31;17(1):2145057. doi: 10.1080/15592324.2022.2145057.

Abstract

Plant glutamate receptor homologs (GLRs), which function as key calcium channels, play pivotal roles in various developmental processes as well as stress responses. The moss Physcomitrium patens, a representative of the earliest land plant lineage, possess multiple pathways of hormone signaling for coordinating growth and adaptation responses. However, it is not clear whether GLRs are connected to hormone-mediated growth control in the moss. In this study, we report that one of the two GLRs in P. patens, PpGLR1, involves in abscisic acid (ABA)-mediated growth regulation. ABA represses the growth of wild-type moss, and intriguingly, the PpGLR1 transcript levels are significantly increased in response to ABA treatment, based on both gene expression and the PpGLR1pro::GUS reporter results. Furthermore, the growth of Ppglr1 knockout moss mutants is hypersensitive to ABA treatment. These results suggest that PpGLR1 plays a critical role in ABA-mediated growth regulation, which provide useful information for our further investigation of the regulatory mechanism between Ca2+ signal and ABA in moss growth control.

Keywords: Glutamate receptor homolog; abscisic acid; gene expression; growth control; moss.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid* / metabolism
  • Abscisic Acid* / pharmacology
  • Bryopsida* / genetics
  • Bryopsida* / metabolism
  • Gene Expression Regulation, Plant / genetics
  • Hormones / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Receptors, Glutamate / genetics
  • Receptors, Glutamate / metabolism

Substances

  • Abscisic Acid
  • Plant Proteins
  • Receptors, Glutamate
  • Hormones

Grants and funding

This work was supported by the Natural Science Foundation of Beijing Municipality (No. 5202003 to D. K. No. 6222001 to C. J.), the Program of Natural Science Foundation of China (No. 32170263 to D. K. No. 31872170 to L. L, No. 31770296 to C. J.), the Key Program of the National Natural Science Foundation of China (No. 31930010 to L.. L), the Key Joint Program of Natural Science and Education Committee Foundation of Beijing (No. KZ202010028049 to L. L), the Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds (No. 19530050165 to L. L.