Generation of cytochrome P450 enzyme-polymorphic human induced pluripotent stem cell lines with CYP2D6*10/CYP3A5*3C and CYP2D6*10/CYP2C19*2

Jaehun Lee; Gyeongmin Kim; Kyun Yoo Chi; Choongseong Han; Jong-Hoon Kim

doi:10.1016/j.scr.2022.102970

Generation of cytochrome P450 enzyme-polymorphic human induced pluripotent stem cell lines with CYP2D610/CYP3A53C and CYP2D610/CYP2C192

Stem Cell Res. 2022 Dec:65:102970. doi: 10.1016/j.scr.2022.102970. Epub 2022 Nov 14.

Authors

Jaehun Lee¹, Gyeongmin Kim², Kyun Yoo Chi², Choongseong Han³, Jong-Hoon Kim⁴

Affiliations

¹ Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea; Department of New Drug Development, NEXEL Co., Ltd., Seoul 02580, South Korea.
² Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea.
³ Department of New Drug Development, NEXEL Co., Ltd., Seoul 02580, South Korea.
⁴ Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea. Electronic address: jhkim@korea.ac.kr.

PMID: 36399926
DOI: 10.1016/j.scr.2022.102970

Abstract

Cytochrome P450 (CYP) reaction phenotyping has become crucial for predicting drug reactions and side effects. Single nucleotide polymorphisms (SNPs) in CYP genes alter drug metabolism capacity and cause unexpected drug-related reactions. Here, we established two human induced pluripotent stem cell (hiPSC) lines with pharmacologically important SNPs in CYP2D6 in conjunction with CYP2C19 or CYP3A5 genes. These hiPSC lines can serve as valuable resources for expanding our understanding of the relationships between genotypes and drug reactions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cytochrome P-450 CYP2C19* / genetics
Cytochrome P-450 CYP2D6* / genetics
Cytochrome P-450 CYP3A* / genetics
Humans
Induced Pluripotent Stem Cells* / enzymology
Pharmaceutical Preparations* / metabolism
Polymorphism, Single Nucleotide

Substances

CYP2C19 protein, human
CYP3A5 protein, human
Cytochrome P-450 CYP2C19
Cytochrome P-450 CYP2D6
Cytochrome P-450 CYP3A
Pharmaceutical Preparations