Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era

Abstract

This chapter describes the core procedures that we have developed over the last two decades to isolate routinely the microglia from postmortem human brains. The method is suitable for brain slices consisting of both gray and white matter.The ability to concomitantly isolate vascular cells with glial cells provides the opportunity to investigate multiple cell types originating from the same donor. This represents a novel approach for -omics research, with the potential for discovering the shared or distinct molecular features among the glia and vascular cells from the same individual.

Keywords: Aging; Human glia; Neurodegeneration; Postmortem; Single cell suspension.