OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice

Muhammad Ilyas; Safdar Hussain Shah; Yasunari Fujita; Kyonoshin Maruyama; Kazuo Nakashima; Kazuko Yamaguchi-Shinozaki; Asad Jan

doi:10.1080/15592324.2022.2142725

OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice

Plant Signal Behav. 2022 Dec 31;17(1):2142725. doi: 10.1080/15592324.2022.2142725.

Authors

Muhammad Ilyas¹, Safdar Hussain Shah¹, Yasunari Fujita², Kyonoshin Maruyama², Kazuo Nakashima², Kazuko Yamaguchi-Shinozaki^{3

4}, Asad Jan¹

Affiliations

¹ Institute of Biotechnology and Genetic Engineering, the University of Agriculture Peshawar, Khyber Pakhtunkhwa, Pakistan.
² Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, Japan.
³ Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
⁴ Research Institute for Agricultural and Life Sciences, Tokyo University of Agriculture, Tokyo, Japan.

Abstract

Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P_OsTZF1:OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The P_OsTZF1:OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The P_OsTZF1:OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in P_OsTZF1:OsTZF1-OX salt-treated plants. Microarray analysis of P_OsTZF1:OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant.

Keywords: CCCH-zinc finger protein; RNA metabolism; oxidative stress; rice; senescence; stress.

MeSH terms

Droughts
Gene Expression Regulation, Plant / genetics
Oryza* / metabolism
Plants, Genetically Modified / metabolism
Sodium Chloride / pharmacology
Zinc Fingers / genetics

Substances

Sodium Chloride

Grants and funding

The author(s) reported that there is no funding associated with the work featured in this article.