Structure and chemopreventive activity of fucoidans from the brown alga Alaria angusta

Anastasia O Zueva; Roza V Usoltseva; Olesya S Malyarenko; Valerii V Surits; Artem S Silchenko; Stanislav D Anastyuk; Anton B Rasin; Huynh Hoang Nhu Khanh; Pham Duc Thinh; Svetlana P Ermakova

doi:10.1016/j.ijbiomac.2022.11.127

Structure and chemopreventive activity of fucoidans from the brown alga Alaria angusta

Int J Biol Macromol. 2023 Jan 15:225:648-657. doi: 10.1016/j.ijbiomac.2022.11.127. Epub 2022 Nov 15.

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Laboratory of Enzyme Chemistry, 159 100-Let Vladivostoku Ave., 690022 Vladivostok, Russian Federation.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Laboratory of Enzyme Chemistry, 159 100-Let Vladivostoku Ave., 690022 Vladivostok, Russian Federation. Electronic address: usoltseva@piboc.dvo.ru.
³ Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang, Viet Nam.

PMID: 36395953
DOI: 10.1016/j.ijbiomac.2022.11.127

Abstract

Six fucoidan fractions were isolated from the brown alga Alaria angusta. Structures of enzymatic hydrolysis products of the fraction 1AaF2 (Fuc:Gal ~ 1:1; 33 % of sulfates) by fucanase from Wenyingzhuangia fucanilytica were studied by chemical and instrumental (NMR spectroscopy and mass-spectrometry) methods. It was shown that 1AaF2 consisted of two structurally different fucoidans: a sulfated 1,3;1,4-α-L-fucan and an enzyme-resistant sulfated and acetylated complex fucogalactan (Fuc:Gal ~ 1:2; 19 % of sulfates) 1AaF2_HMP containing extended 1,3-linked fucose and 1,3/1,4-linked galactose fragments (up to 5 residues). The fractions 1AaF2 and 1AaF2_HMP were a non-cytotoxic, possessed dose-dependent chemopreventive effect on EGF-induced neoplastic cell transformation of mouse normal epidermal JB6 Cl41 cells and inhibited the colony formation of human melanoma SK-MEL-28 cells.

Keywords: Alaria angusta; Brown alga; Chemopreventive activity; Enzymatic transformation; Fucoidan; Structure.

MeSH terms

Animals
Antineoplastic Agents* / chemistry
Antineoplastic Agents* / pharmacology
Humans
Melanoma*
Mice
Phaeophyceae* / chemistry
Polysaccharides / chemistry
Polysaccharides / pharmacology
Sulfates / chemistry

Substances

fucoidan
Antineoplastic Agents
Polysaccharides
Sulfates