Monitoring Gut Epithelium Serotonin and Melatonin Overflow Provides Spatial Mapping of Inflammation

Fernando Perez; Nikki Kotecha; Brigitte Lavoie; Gary M Mawe; Bhavik Anil Patel

doi:10.1002/cbic.202200334

Monitoring Gut Epithelium Serotonin and Melatonin Overflow Provides Spatial Mapping of Inflammation

Chembiochem. 2023 Jan 17;24(2):e202200334. doi: 10.1002/cbic.202200334. Epub 2022 Dec 2.

Authors

Fernando Perez¹, Nikki Kotecha¹, Brigitte Lavoie², Gary M Mawe², Bhavik Anil Patel¹

Affiliations

¹ Centre for Stress and Age-Related Disease, School of Applied Sciences, University of Brighton, Brighton, BN2 4GJ, UK.
² Department of Neurological Sciences, The University of Vermont, Burlington, VT-05405, USA.

Abstract

Electrochemical arrays were used to measure the overflow of serotonin (5-HT) and melatonin (MEL) from the entire colon of healthy mice and mice with chemical-induced inflammatory bowel disease (IBD), to understand the interplay between inflammation and colonic function. We show that 5-HT overflow is increased, whilst MEL levels are reduced, in inflamed tissues. The levels of MEL are increased at the interface between healthy and inflamed regions within the colon and may limit the spread of inflammation. Understanding the interplay between inflammation and mucosal epithelial signalling can provide key insight into colonic function and aid the development of effective therapeutic strategies to treat gastrointestinal diseases.

Keywords: EC cells; electrode arrays; inflammation; melatonin; serotonin.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Epithelium
Inflammation
Intestinal Mucosa
Melatonin*
Mice
Serotonin*

Substances

Serotonin
Melatonin

Abstract

Publication types

MeSH terms

Substances

Grants and funding