Comparative transcriptomics and bioinformatics analysis of genes related to photosynthesis in Eucalyptus camaldulensis

Ni Zhan; Liejian Huang; Zhen Wang; Yaojian Xie; Xiuhua Shang; Guo Liu; Zhihua Wu

doi:10.7717/peerj.14351

Comparative transcriptomics and bioinformatics analysis of genes related to photosynthesis in Eucalyptus camaldulensis

PeerJ. 2022 Nov 11:10:e14351. doi: 10.7717/peerj.14351. eCollection 2022.

Authors

Ni Zhan^{1

2}, Liejian Huang³, Zhen Wang², Yaojian Xie¹, Xiuhua Shang¹, Guo Liu¹, Zhihua Wu¹

Affiliations

¹ Research Institute of Fast-growing Trees, Chinese Academy of Forestry, Zhanjiang, Guangdong, China.
² Langfang Normal University, Langfang, Hebei, China.
³ Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, China.

Abstract

The timber species Eucalyptus camaldulensis is one of the most important in southern China. Therefore, it is essential to understand the photosynthetic pattern in eucalyptus leaves. In the present study, eighteen photosynthesis-related genes were analyzed using bioinformatics methods. The results indicated that there were ten differentially expressed ribose-5-phosphate isomerase genes (RPI), and six of them were up-regulated in the mature leaves compared to the young leaves, while others were down-regulated. The differential expression of four rubisco methyltransferase genes (RBCMT) were observed. Two of them were up-regulated, while two were down-regulated in mature leaves compared to young leaves. Furthermore, two ribulose-phosphate-3-epimerase genes (RPE) were up-regulated in the mature leaves compared to the young leaves. In contrast, two genes involved in triosephosphate isomerase (TIM) were down-regulated in mature leaves compared with young leaves. The current study provides basic information about the transcriptome of E. camaldulensis and lays a foundation for further research in developing and utilizing important photosynthetic genes.

Keywords: Eucalyptus camaldulensis; Gene analysis; Photosynthesis; Transcriptional sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Eucalyptus* / genetics
Photosynthesis / genetics
Plant Leaves / genetics
Transcriptome / genetics

Grants and funding

This study was supported by the National Natural Science Foundation of China (grant number 31570615); the Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (grant number CAFYBB2019MA006); and the Fundamental Research Funds for the Central Non-Profit Research Institution of CAF (grant number CAFYBB2017ZX001-5). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.