Plasma-Based Metabolomics Profiling of High-Risk Human Papillomavirus and their Emerging Roles in the Progression of Cervical Cancer

Aozheng Chen; Min Xu; Jing Chen; Tingting Chen; Qin Wang; Runjie Zhang; Jin Qiu

doi:10.1155/2022/6207701

Plasma-Based Metabolomics Profiling of High-Risk Human Papillomavirus and their Emerging Roles in the Progression of Cervical Cancer

Biomed Res Int. 2022 Nov 3:2022:6207701. doi: 10.1155/2022/6207701. eCollection 2022.

Authors

Aozheng Chen^{1

2}, Min Xu^{1

2}, Jing Chen², Tingting Chen², Qin Wang^{1

2}, Runjie Zhang^{1

2}, Jin Qiu²

Affiliations

¹ Hongqiao International Institute of Medicine, China.
² Obstetrics and Gynecology Department, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No. 1111, XianXia Road, Shanghai 200336, China.

Abstract

High-risk human papillomavirus (HR-HPV) is the main etiological factor for cervical cancer. Accumulating evidence has suggested the active role of metabolites in the initiation and progression of cancers. This study explored the plasma metabolic profiles of HPV-16 positive (HPV16 (+)), HPV-18 positive (HPV18 (+)), and HPV negative (CTL) individuals using a nontargeted metabolomics approach. C8 ceramide-1-Phosphate (d18 : 1/8 : 0) was found to inhibit cervical cancer cell proliferation and migration in vitro, evidenced by CCK8 experiments, a cell migration test, RT-qPCR, and western blotting. The underlying mechanism demonstrated that C8 inhibited proliferation and migration in cervical cancer cells via the MAPK/JNK1 signaling pathway. These findings may contribute to the clinical treatment of HR-HPV-induced cervical cancer by intervening in its initiation and progression.

MeSH terms

Cervix Uteri
Female
Human papillomavirus 16
Humans
Metabolomics
Papillomavirus Infections* / complications
Uterine Cervical Neoplasms*