Fructooligosaccharides (FOS) significantly increased the relative abundance of intestinal B. pseudolongum in mice with different genotypes

Jiayu Gu; Shumao Cui; Xin Tang; Zhenmin Liu; Jianxin Zhao; Hao Zhang; Bingyong Mao; Wei Chen

doi:10.1016/j.crfs.2022.10.030

Fructooligosaccharides (FOS) significantly increased the relative abundance of intestinal B. pseudolongum in mice with different genotypes

Curr Res Food Sci. 2022 Nov 4:5:2178-2189. doi: 10.1016/j.crfs.2022.10.030. eCollection 2022.

Authors

Jiayu Gu^{1

2}, Shumao Cui^{1

2}, Xin Tang^{1

2}, Zhenmin Liu³, Jianxin Zhao^{1

2}, Hao Zhang^{1

2

4}, Bingyong Mao^{1

2}, Wei Chen^{1

2

4}

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
² School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
³ State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co.,Ltd, Shanghai, 200436, PR China.
⁴ National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, 214122, PR China.

Abstract

Fructooligosaccharides (FOS) promote the proliferation of Bifidobacterium, especially Bifidobacterium pseudolongum in C57BL/6J mice. However, the response of intestinal microbes to FOS is influenced by host genotypes. Therefore, we compared the intestinal microbiota of four commonly used mice before and after FOS intervention, including C57BL/6J, BALB/c, Institute Cancer Research (ICR), and Kunming (KM) mice. The intestinal microbiota of the four genotypes exhibited similarities in composition but differences in relative abundance. Bifidobacterium was significantly increased to different degrees in the four genotypes of mice after FOS intervention, and Akkermansia and Bacteroides were also significantly increased in BALB/c and KM mice. Lactobacillus and Alistipes levels were unchanged or decreased. Within the genus Bifidobacterium, B. pseudolongum was the dominant species in the four genotypes of mice and proliferated significantly after FOS intervention, with dramatic proliferation in C57BL/6J mice (9.49%). Furthermore, eight strains of B. pseudolongum were screened from the feces of mice with four genotypes, and there was a great difference in the ability and manner of utilizing FOS among the strains. The strains from C57BL/6J mice exhibited the strongest utilization of 1-kestose (GF2), whereas other strains could utilize both GF2 and nistose (GF3) weakly. The gut microbial analysis of mice with different genotypes complemented our previous studies. The results provided the background strains of the different mouse genotypes and suggested a correlation between the utilization ability and the response of the strains to FOS. Further studies on the utilization ability of strains and competition in the intestine will contribute to the understanding of the mechanisms of the intestinal microbial response to diet.

Keywords: B. pseudolongum; FOS; Genotypes; Intestinal microbiota.