DANAMIC: Data analyzer of minimum inhibitory concentrations - Protocol to analyze antimicrobial susceptibility data

Javier Garcia-Ruiz; Maxim G Ryadnov

doi:10.1016/j.xpro.2022.101782

DANAMIC: Data analyzer of minimum inhibitory concentrations - Protocol to analyze antimicrobial susceptibility data

STAR Protoc. 2022 Nov 4;3(4):101782. doi: 10.1016/j.xpro.2022.101782. eCollection 2022 Dec 16.

Authors

Javier Garcia-Ruiz¹, Maxim G Ryadnov²

Affiliations

¹ National Physical Laboratory, Teddington, Middlesex TW11 0LW, UK.
² National Physical Laboratory, Teddington, Middlesex TW11 0LW, UK; Department of Physics, King's College London, Strand Lane, London WC2R 2LS, UK. Electronic address: max.ryadnov@npl.co.uk.

Abstract

This protocol describes an open-source software developed to analyze experimental data obtained using antimicrobial susceptibility assays. We first describe experimental procedures for testing the activity of antimicrobial agents in vitro based on reference standards (BS EN ISO 20776-1:2020). We then describe the software protocol to analyze and convert the data obtained using these procedures into minimum inhibitory concentrations. This approach enables automated data analysis for microdilution assays and can be adapted for high-throughput antimicrobial screening.

Keywords: Bioinformatics; Cell culture; Microbiology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents* / pharmacology
Anti-Infective Agents* / pharmacology
Microbial Sensitivity Tests
Reference Standards

Substances

Anti-Bacterial Agents
Anti-Infective Agents