Using a zebrafish model to understand adherent-invasive Escherichia coli infection

Dhrubajyoti Nag; Dustin Farr; Saumya Raychaudhuri; Jeffrey H Withey

doi:10.1016/j.xpro.2022.101810

Using a zebrafish model to understand adherent-invasive Escherichia coli infection

STAR Protoc. 2022 Nov 2;3(4):101810. doi: 10.1016/j.xpro.2022.101810. eCollection 2022 Dec 16.

Authors

Dhrubajyoti Nag¹, Dustin Farr¹, Saumya Raychaudhuri², Jeffrey H Withey³

Affiliations

¹ Department of Biochemistry, Microbiology, and Immunology, Wayne State University School of Medicine, Detroit, MI, USA.
² CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.
³ Department of Biochemistry, Microbiology, and Immunology, Wayne State University School of Medicine, Detroit, MI, USA. Electronic address: jwithey@med.wayne.edu.

Abstract

A zebrafish model was developed to study AIEC colonization, invasion, and inflammation. This model can also be used to study the beneficial effects of a probiotic on AIEC infection of adult zebrafish. Bacteria are grown in vitro and then fish are infected with AIEC by immersion. Subsequently, colonization and inflammation can be assessed. Exposing fish to probiotic at different time points relative to AIEC can determine beneficial effects of probiotics as prophylactics or therapeutics against AIEC. For complete details on the use and execution of this protocol, please refer to Nag et al. (2022).

Keywords: Immunology; Microbiology; Model organisms; Molecular biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Adhesion
Escherichia coli
Escherichia coli Infections* / therapy
Inflammation / microbiology
Zebrafish*