Enhancing gene editing and gene targeting efficiencies in Arabidopsis thaliana by using an intron-containing version of ttLbCas12a

Plant Biotechnol J. 2023 Mar;21(3):457-459. doi: 10.1111/pbi.13964. Epub 2022 Nov 30.

Authors

Patrick Schindele¹, Laura Merker¹, Tom Schreiber², Anja Prange², Alain Tissier², Holger Puchta¹

Affiliations

¹ Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany.
² Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany.

No abstract available

Keywords: CRISPR/Cas12a; gene editing; gene targeting; intron; ttLbCas12a.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis* / genetics
CRISPR-Cas Systems
Gene Editing*
Gene Targeting
Introns