IFI204 protects host defense against Staphylococcus aureus-induced pneumonia by promoting extracellular traps formation

Exp Cell Res. 2023 Jan 1;422(1):113415. doi: 10.1016/j.yexcr.2022.113415. Epub 2022 Nov 13.

Abstract

Interferon-inducible protein 204 (IFI204) is an intracellular DNA receptor that can recognize DNA viruses and intracellular bacteria. Extracellular traps (ETs) have been recognized as an indispensable antimicrobial barrier that play an indispensable role in bacterial, fungal, parasitic, and viral infections. However, how ETs form and the mechanisms by which IFI204 function in Staphylococcus aureus pneumonia are still unclear. Moreover, by in vitro experiments, we proved that IFI204 deficiency decreases the formation of ETs induced by Staphylococcus aureus in a NOX-independent manner. More importantly, Deoxyribonuclease I (DNase I) treatment significantly inhibited the formation of ETs. IFI204 contributed to ETs formation by promoting citrullination of histone H3 and the expression of PAD4 (peptidylarginine deiminase 4). Altogether, these findings highlight the potential importance of IFI204 for host defense against S. aureus USA300-TCH1516 infection.

Keywords: Extracellular traps; IFI204; PAD4; Pneumonia; Staphylococcus aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Extracellular Traps* / genetics
  • Extracellular Traps* / metabolism
  • Interferons / metabolism
  • Mice
  • Neutrophils / metabolism
  • Pneumonia, Staphylococcal* / genetics
  • Pneumonia, Staphylococcal* / metabolism
  • Staphylococcus aureus

Substances

  • Interferons
  • Ifi205 protein, mouse