Purification of Thymocyte and T Cell Subsets

Andrea C Carpenter; Laura B Chopp; Jong Kyong Kim; Rémy Bosselut

doi:10.1007/978-1-0716-2740-2_8

Purification of Thymocyte and T Cell Subsets

Methods Mol Biol. 2023:2580:151-163. doi: 10.1007/978-1-0716-2740-2_8.

Authors

Andrea C Carpenter¹, Laura B Chopp², Jong Kyong Kim³, Rémy Bosselut⁴

Affiliations

¹ Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
² Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
³ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
⁴ Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. bosselur@mail.nih.gov.

Abstract

Many analytical or cell culture procedures require homogeneous starting cell populations that cannot be obtained directly from organ dissection. Here, we describe two enrichment procedures to achieve this goal and discuss their respective advantages in specific experimental contexts.

Keywords: Flow cytometric sorting; Magnetic bead depletion; Single-cell suspension; T cell purification.

MeSH terms

Flow Cytometry / methods
T-Lymphocyte Subsets*
Thymocytes*

Grants and funding

Z01 BC010671/ImNIH/Intramural NIH HHS/United States