Apparent thermal acclimation of soil heterotrophic respiration mainly mediated by substrate availability

Abstract

Multiple lines of existing evidence suggest that increasing CO₂ emission from soils in response to rising temperature could accelerate global warming. However, in experimental studies, the initial positive response of soil heterotrophic respiration (R_H ) to warming often weakens over time (referred to apparent thermal acclimation). If the decreased R_H is driven by thermal adaptation of soil microbial community, the potential for soil carbon (C) losses would be reduced substantially. In the meanwhile, the response could equally be caused by substrate depletion, and would then reflect the gradual loss of soil C. To address uncertainties regarding the causes of apparent thermal acclimation, we carried out sterilization and inoculation experiments using the soil samples from an alpine meadow with 6 years of warming and nitrogen (N) addition. We demonstrate that substrate depletion, rather than microbial adaptation, determined the response of R_H to long-term warming. Furthermore, N addition appeared to alleviate the apparent acclimation of R_H to warming. Our study provides strong empirical support for substrate availability being the cause of the apparent acclimation of soil microbial respiration to temperature. Thus, these mechanistic insights could facilitate efforts of biogeochemical modeling to accurately project soil C stocks in the future climate.

Keywords: heterotrophic respiration; microbial adaptation; nitrogen addition; substrate depletion; warming.