Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance

Eriko Aoyama; Masaharu Takigawa

doi:10.1007/978-1-0716-2744-0_7

Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance

Methods Mol Biol. 2023:2582:77-86. doi: 10.1007/978-1-0716-2744-0_7.

Authors

Eriko Aoyama¹, Masaharu Takigawa²

Affiliations

¹ Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, Okayama, Japan. eaoyama@cc.okayama-u.ac.jp.
² Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, Okayama, Japan.

PMID: 36370345
DOI: 10.1007/978-1-0716-2744-0_7

Abstract

CCN proteins are known to bind to various growth factors, cytokines, and membrane proteins. Since these bindings are closely involved in the function of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simplicity and cost advantage, and a surface plasmon resonance assay, which can determine the binding affinities between CCN proteins and their partners.

Keywords: CCN2; SPR; binding proteins; peptide; solid-phase binding assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay
Intercellular Signaling Peptides and Proteins*
Membrane Proteins
Protein Binding
Surface Plasmon Resonance*

Substances

Intercellular Signaling Peptides and Proteins
Membrane Proteins