Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments

Inês Rosado Vitorino; Dominika Klimek; Magdalena Calusinska; Alexandre Lobo-da-Cunha; Vítor Vasconcelos; Olga Maria Lage

doi:10.3390/microorganisms10112151

Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments

Microorganisms. 2022 Oct 30;10(11):2151. doi: 10.3390/microorganisms10112151.

Authors

Inês Rosado Vitorino^{1

2}, Dominika Klimek^{3

4}, Magdalena Calusinska³, Alexandre Lobo-da-Cunha⁵, Vítor Vasconcelos^{1

2}, Olga Maria Lage^{1

2}

Affiliations

¹ Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
² CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal.
³ The Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), 41 rue du Brill, L-4422 Belvaux, Luxembourg.
⁴ The Faculty of Science, Technology and Medicine (FSTM), University of Luxembourg, 2 Avenue de l'Université, L-4365 Esch-sur-Alzette, Luxembourg.
⁵ Laboratório de Biologia Celular, Instituto de Ciências Biomédicas Abel Salazar, ICBAS, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.

Abstract

The phylum Planctomycetota is known for having uncommon biological features. Recently, biotechnological applications of its members have started to be explored, namely in the genus Stieleria. Here, we formally describe a novel Stieleriaisolate designated as strain ICT_E10.1^T, obtained from sediments collected in the Tagus estuary (Portugal). Strain ICT_E10.1^T is pink-pigmented, spherical to ovoid in shape, and 1.7 µm ± 0.3 × 1.4 µm ± 0.3 in size. Cells cluster strongly in aggregates or small chains, divide by budding, and have prominent fimbriae. Strain ICT_E10.1^T is heterotrophic and aerobic. Growth occurs from 20 to 30 °C, from 0.5 to 3% (w/v) NaCl, and from pH 6.5 to 11.0. The analysis of the 16S rRNA gene sequence placed strain ICT_E10.1^T into the genus Stieleria with Stieleria neptunia Enr13^T as the closest validly described relative. The genome size is 9,813,311 bp and the DNA G+C content is 58.8 mol%. Morphological, physiological, and genomic analyses support the separation of this strain into a novel species, for which we propose the name Stieleria sedimenti represented by strain ICT_E10.1^T as the type of strain (=CECT 30514^T= DSM 113784^T). Furthermore, this isolate showed biotechnological potential by displaying relevant biosynthetic gene clusters and potent activity against Staphylococcus aureus.

Keywords: Alcochete; Planctomycetota; bioactivity screenings; biosynthetic gene clusters; carbohydrate-active enzymes; iChip; novel taxa; organic extraction; transmission electron microscopy.

Abstract

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