Association of Long Noncoding RNA HOTAIR Polymorphism and the Clinical Manifestations of Diabetic Retinopathy

Chih-Chun Chuang; Kai Wang; Yi-Sun Yang; Edy Kornelius; Chih-Hsin Tang; Chia-Yi Lee; Hsiang-Wen Chien; Shun-Fa Yang

doi:10.3390/ijerph192114592

Association of Long Noncoding RNA HOTAIR Polymorphism and the Clinical Manifestations of Diabetic Retinopathy

Int J Environ Res Public Health. 2022 Nov 7;19(21):14592. doi: 10.3390/ijerph192114592.

Authors

Chih-Chun Chuang^{1

2

3}, Kai Wang^{4

5

6}, Yi-Sun Yang^{7

8}, Edy Kornelius^{7

8}, Chih-Hsin Tang^{9

10}, Chia-Yi Lee^{1

11}, Hsiang-Wen Chien^{4

5

6

12}, Shun-Fa Yang^{1

13}

Affiliations

¹ Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
² Department of Ophthalmology, Changhua Christian Hospital, Changhua 500, Taiwan.
³ Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung 402, Taiwan.
⁴ Department of Ophthalmology, Cathay General Hospital, Taipei 106, Taiwan.
⁵ Departments of Ophthalmology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan.
⁶ School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
⁷ School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.
⁸ Department of Internal Medicine, Division of Endocrinology and Metabolism, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
⁹ School of Medicine, China Medical University, Taichung 404, Taiwan.
¹⁰ Department of Medical Laboratory Science and Biotechnology, College of Medical and Health Science, Asia University, Taichung 413, Taiwan.
¹¹ Department of Ophthalmology, Nobel Eye Institute, Taipei 115, Taiwan.
¹² School of Medicine, National Tsing Hua University, Hsinchu 300, Taiwan.
¹³ Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402, Taiwan.

Abstract

The aim of the current study is to evaluate the possible correlation between the single-nucleotide polymorphisms (SNP) of HOX transcript antisense intergenic RNA (HOTAIR) and the clinical characteristics of diabetic retinopathy (DR). Four loci of HOTAIR SNPs, including rs920778 (T/C), rs12427129 (C/T), rs4759314 (A/G), and rs1899663 (G/T), were genotyped via the TaqMan allelic discrimination for 276 DR individuals and 452 non-DR patients. The distribution frequency of HOTAIR SNP rs12427129 CT [adjusted odds ratio (AOR): 1.571, 95% CI: 1.025-2.408, p = 0.038], HOTAIR SNP rs12427129 CT+TT (AOR: 1.611, 95% CI: 1.061-2.446, p = 0.025), and HOTAIR SNP rs1899663 TT (AOR: 2.443, 95% CI: 1.066-5.595, p = 0.035) were significantly higher in the DR group. Moreover, the proliferative diabetic retinopathy (PDR) subgroup revealed a significantly higher distribution of HOTAIR SNP rs12427129 CT+TT (AOR: 2.016, 95% CI: 1.096-3.710, p = 0.024) and HOTAIR SNP rs1899663 TT (AOR: 4.693, 95% CI: 1.765-12.479, p = 0.002), and the distribution frequencies of HOTAIR SNP rs12427129 CT (AOR: 3.722, 95% CI: 1.555-8.909, p = 0.003), HOTAIR SNP rs12427129 CT+TT (AOR: 4.070, 95% CI: 1.725-9.600, p = 0.001), and HOTAIR SNP rs1899663 TT (AOR: 11.131, 95% CI: 1.521-81.490, p = 0.018) were significantly higher in the female PDR subgroup. Regarding the clinical characters, the DR patients with HOTAIR SNP rs1899663 GT+TT revealed a significantly shorter duration of diabetes compared to the DR patients with HOTAIR SNP rs1899663 GG (10.54 ± 8.19 versus 12.79 ± 7.73, p = 0.024). In conclusion, HOTAIR SNP rs12427129 and rs1899663 are strongly correlated to the presence of DR, especially for a female with PDR.

Keywords: EGFR mutation; GAS5; lung cancer; polymorphism.

MeSH terms

Case-Control Studies
Diabetes Mellitus*
Diabetic Retinopathy* / genetics
Female
Genetic Predisposition to Disease
Genotype
Humans
Polymorphism, Single Nucleotide
RNA, Long Noncoding* / genetics

Substances

RNA, Long Noncoding
HOTAIR long untranslated RNA, human

Grants and funding

This research received no external funding.