Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity

Shuzhen Zhao; Jie Sun; Jinbo Sun; Xiaoqian Zhang; Chuanzhi Zhao; Jiaowen Pan; Lei Hou; Ruizheng Tian; Xingjun Wang

doi:10.3390/genes13112076

Insights into the Novel FAD2 Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity

Genes (Basel). 2022 Nov 9;13(11):2076. doi: 10.3390/genes13112076.

Authors

Shuzhen Zhao^{1

2}, Jie Sun^{1

2}, Jinbo Sun¹, Xiaoqian Zhang^{1

2}, Chuanzhi Zhao¹, Jiaowen Pan¹, Lei Hou^{1

2}, Ruizheng Tian¹, Xingjun Wang^{1

2}

Affiliations

¹ Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China.
² College of Life Sciences, Shandong Normal University, Jinan 250014, China.

Abstract

AhFAD2 is a key enzyme catalyzing the conversion of oleic acid into linoleic acid. The high oleic acid characteristic of peanut mainly comes from the homozygous recessive mutation of AhFAD2A and AhFAD2B genes (aabb). However, even in high-oleic-acid varieties with the aabb genotype, the oleic acid content of seeds with different maturity varies significantly. Therefore, in addition to AhFAD2A and AhFAD2B, other FAD2 members or regulators may be involved in this process. Which FAD2 genes are involved in the regulatory processes associated with seed maturity is still unclear. In this study, four stable lines with different genotypes (AABB, aaBB, AAbb, and aabb) were used to analyze the contents of oleic acid and linoleic acid at different stages of seed development in peanut. Three new AhFAD2 genes (AhFAD2-7, AhFAD2-8, and AhFAD2-9) were cloned based on the whole-genome sequencing results of cultivated peanuts. All peanut FAD2 genes showed tissue preference in expression; however, only the expression level of AhFAD2-7 was positively correlated with the linoleic acid concentration in peanut seeds. These findings provide new insights into the regulation of oleic acid accumulation by maturity, and AhFAD2-7 plays an important role in the maturity dependent accumulation of oleic acid and linoleic acid in peanut.

Keywords: FAD2; oleic acid; peanut; seed maturity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arachis*
Fatty Acid Desaturases / genetics
Linoleic Acid / metabolism
Oleic Acid* / metabolism
Seeds

Substances

Oleic Acid
Fatty Acid Desaturases
Linoleic Acid

Grants and funding

This work was supported by Key Research and Development Project of Shandong Province (2022LZGC007), Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences, the National Natural Science Foundation of China (31861143009; 32072090) and Taishan Scholar Project of Shandong Province (ts20190964).