A stimuli-responsive colorimetric aptasensor based on the DNA hydrogel-coated MOF for fumonisin B1 determination in food samples

Yuhan Sun; Yan Lv; Yin Zhang; Zhouping Wang

doi:10.1016/j.foodchem.2022.134242

A stimuli-responsive colorimetric aptasensor based on the DNA hydrogel-coated MOF for fumonisin B₁ determination in food samples

Food Chem. 2023 Mar 1:403:134242. doi: 10.1016/j.foodchem.2022.134242. Epub 2022 Sep 15.

Authors

Yuhan Sun¹, Yan Lv¹, Yin Zhang², Zhouping Wang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.

PMID: 36358091
DOI: 10.1016/j.foodchem.2022.134242

Abstract

Fumonisin B₁ (FB₁), the most occurring fumonisin, is capable of disrupting sphingolipid metabolism and poses a serious threat to human and animal health. The high incidence and toxicity of FB₁ have created an urgent need for alternative quantitative determination strategies. Herein, a colorimetric aptasensor was developed by combining the catalytical metal-organic frameworks with the polyacrylamide-DNA hydrogel. The porphyrin-based MOF provided the aptasensor with robust peroxidase-like activity while maintaining good stability. Concurrently, the polyacrylamide-DNA hydrogel constructed via strand-induced hybridization chain reaction (HCR) endowed the aptasensor with outstanding stimuli-responsiveness towards FB₁ molecules. The developed aptasensor demonstrated remarkable selectivity and ease of use for assays conducted in solution and food samples. It also exhibited superior analytical performance with a linear range of 0.05-100 ng mL^-1 and a limit of detection (LOD) of 0.024 ng mL^-1. The strategy could serve as a model to develop diverse stimuli-responsive aptasensors targeting hazards in food safety.

Keywords: Aptasensor; DNA hydrogel; Fumonisin B(1); Metal-organic frameworks.

MeSH terms

Animals
Aptamers, Nucleotide* / genetics
Biosensing Techniques*
Colorimetry
DNA
Fumonisins* / analysis
Humans
Hydrogels
Limit of Detection

Substances

fumonisin B1
Fumonisins
Hydrogels
DNA
Aptamers, Nucleotide