Effects of dietary trans-cinnamaldehyde on growth performance, lipid metabolism, immune response and intestinal microbiota of Litopenaeus vannamei

Zhifei Chen; Futao Jing; Mingxiang Lu; Chen Su; Ruixue Tong; Luqing Pan

doi:10.1016/j.fsi.2022.11.008

Effects of dietary trans-cinnamaldehyde on growth performance, lipid metabolism, immune response and intestinal microbiota of Litopenaeus vannamei

Fish Shellfish Immunol. 2022 Dec:131:908-917. doi: 10.1016/j.fsi.2022.11.008. Epub 2022 Nov 8.

Authors

Zhifei Chen¹, Futao Jing², Mingxiang Lu¹, Chen Su¹, Ruixue Tong¹, Luqing Pan³

Affiliations

¹ The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.
² Shandong Fisheries Development and Resources Conservation Center, Jinan, 50000, China.
³ The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China. Electronic address: panlq@ouc.edu.cn.

PMID: 36356856
DOI: 10.1016/j.fsi.2022.11.008

Abstract

The purpose of this study was to evaluate the effects of dietary trans-cinnamaldehyde (TC) on growth performance, lipid metabolism, immune response and intestinal microbiota of Litopenaeus vannamei. Shrimp were randomly divided into 4 groups, with 3 replicants in each group and 70 shrimp in each replicant. The contents of TC in the four groups were 0, 0.4, 0.8 and 1.2 g kg^-1, respectively. Samples were taken after 56 days, followed by a 7-day vibrio harveyi challenge experiment. The results showed that TC significantly improved the growth performance by enhancing the activity of digestive enzymes in shrimp (P < 0.05). TC also reduced the content of crude fat (P < 0.05). The addition of TC to the diet attenuated lipid deposition, as evidenced by a reduction in the content of crude fat and a decrease in plasma levels of cholesterol and triglycerides (P < 0.05). The expression of key genes for fatty acid and triglycerides synthesis were significantly down-regulated and key genes for fatty acid β-oxidation were significantly up-regulated (P < 0.05). In addition, the immune response and antioxidant capability of shrimp were significantly enhanced by the addition of TC to the diet (P < 0.05). Meanwhile, TC could improve intestinal health by increasing the abundance of beneficial bacteria and decreasing the abundance of pathogenic bacteria, but had no significant effect on alpha diversity and beta diversity (P > 0.05). In addition, the results of histopathological sections and plasma transaminase studies showed that TC could improve the health status of hepatopancreas and was a safe nutritional supplement. After the 7-day Vibrio harveyi challenge, the cumulative mortality of shrimp decreased with increasing levels of dietary TC compared with control group (P < 0.05). These results suggested that TC could be used as a nutritional supplement for shrimp to enhance disease resistance and reduce lipid accumulation.

Keywords: Growth performance; Immune response; Intestinal microbiota; Lipid metabolism; Litopenaeus vannamei; Trans-cinnamaldehyde.

Publication types

Randomized Controlled Trial, Veterinary

MeSH terms

Animal Feed / analysis
Animals
Diet / veterinary
Fatty Acids / metabolism
Gastrointestinal Microbiome*
Immunity, Innate
Lipid Metabolism
Lipids
Penaeidae*
Triglycerides / metabolism

Substances

cinnamaldehyde
Fatty Acids
Triglycerides
Lipids

Supplementary concepts

Vibrio harveyi