Aflatoxin B1 administration induces reactive oxygen species production and apoptosis of erythrocytes in mice

Abstract

Aflatoxin is a naturally occurring mycotoxin that has various toxic effects to humans and various other animals. In the current study, we have investigated the toxic effects of Aflatoxin B1 (AFB1) on erythrocytes in the blood circulation of mice. Mice were administered orally with repeated doses of AFB1 (0.3 mg/kg of body weight three times a week for four weeks). AFB1 administration resulted in sustained anemia and a significant reduction in blood erythrocyte number as well as hemoglobin level was seen at different time schedules. Body weight, erythrocyte count, and Hb were significantly decreased on days 30 and 45 post-AFB1 administration. The reticulocytes proportion in circulation was analyzed by staining the cells with anti-mouse CD71 monoclonal antibody and flow cytometric analysis. The ROS level and apoptotic cell proportion were determined by staining with CM-H₂DCFDA and Annexin V antibody. AFB1 treatment leads to an increment in reticulocytes production. A significant increase in reactive oxygen species (ROS) and apoptotic cells were also observed in erythrocytes.

Keywords: Aflatoxin; Anemia; Apoptosis; Erythrocytes; Oxidative stress.