Fast, specific, and ultrasensitive antibiotic residue detection by monolayer WS2-based field-effect transistor sensor

Xiaojie Wei; Chengbin Liu; Hehe Qin; Ziwei Ye; Xinru Liu; Boyang Zong; Zhuo Li; Shun Mao

doi:10.1016/j.jhazmat.2022.130299

Fast, specific, and ultrasensitive antibiotic residue detection by monolayer WS₂-based field-effect transistor sensor

J Hazard Mater. 2023 Feb 5;443(Pt B):130299. doi: 10.1016/j.jhazmat.2022.130299. Epub 2022 Nov 1.

Authors

Xiaojie Wei¹, Chengbin Liu², Hehe Qin¹, Ziwei Ye¹, Xinru Liu¹, Boyang Zong¹, Zhuo Li¹, Shun Mao³

Affiliations

¹ College of Environmental Science and Engineering, Biomedical Multidisciplinary Innovation Research Institute, Shanghai East Hospital, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
² Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
³ College of Environmental Science and Engineering, Biomedical Multidisciplinary Innovation Research Institute, Shanghai East Hospital, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address: shunmao@tongji.edu.cn.

PMID: 36356526
DOI: 10.1016/j.jhazmat.2022.130299

Abstract

Antibiotic residues cause increasing concern in environmental ecology and public health, which needs efficient analysis strategy for monitoring and control. In this study, a fast, specific, and ultrasensitive sensor based on field-effect transistor (FET) has been proposed for the detection of ampicillin (AMP). The sensor involves monolayer tungsten disulfide (WS₂) nanosheet as the sensing channel, single-stranded DNA (ssDNA) as the sensing probe, and gold nanoparticle (Au NP) as the linker. The WS₂/Au/ssDNA FET sensor responds rapidly to AMP in a wide linear detection range (10^-12-10^-6 M) and has low limit of detection (0.556 pM), which meets the permissible standards of AMP in water and food. The sensing mechanism study suggests that the excellent sensor response results from the increased number of negative charges in the Debye length and the consequent accumulation of holes in WS₂ channel after the addition of AMP. Moreover, satisfactory sensing performance was confirmed in real water samples, indicating the potential application of the proposed method in practical AMP detection. The reported FET sensing strategy provides new insights in antibiotic analysis for risk assessment and control.

Keywords: Ampicillin; Antibiotic detection; Aptamer DNA; Field-effect transistor; Monolayer WS(2).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Monophosphate
Anti-Bacterial Agents / chemistry
Biosensing Techniques* / methods
Gold / chemistry
Metal Nanoparticles* / chemistry
Water

Substances

tungsten sulfide
Anti-Bacterial Agents
Gold
Water
Adenosine Monophosphate